Little Ouseburn is a small village and civil parish in the Harrogate district of North Yorkshire, England. It is situated near the A1 road motorway and 6 miles south-east of Boroughbridge. It consists of two roads, Main Street which is the residential area, and Church Lane which contains a Holy Trinity Church that is a grade I listed building. It also has a small brick bridge over a stream which leads to a new road which leads to Great Ouseburn. According to the 2011 census data the total population of Little Ouseburn is 264.